Hemoglobin Brockton [beta 138 (H16) Ala—-Professional] has very low oxygen affinity but electrophoretic mobility and hemolysate binding are typical, suggesting that the substitution at beta 138 won't disrupt critical polar interactions, including the hydrogen bonds and salt bridges concerning Asp 137 and Pro 138, that add towards the cooperative oxygen-binding process in Hb A. The variant is unstable and has become connected with mild anemia.
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